There was a close one, and one that actually did it for me, both of which are very late in the series.
The close one was at the end of Ep 7. After the tea party, when Battler finally shows up to console the crying Ange. It chokes me up.
But the one that actually got me, and messed me up inside, was during Ep 8. And it’s probably not a scene that you would expect to bring someone to tears. The goat battle, in itself, isn’t an emotional scene in that way, of course, but during the fight on first read it was pretty clear to me that the goats were voicing a lot of the fan and online reactions to various characters and developments in Umineko. And of course, the goats, the ‘audience inserts’ that are saying things that we the readers probably have at some point, are written as stupid, destructive, horrible people destroying the ground they stand on. And I started thinking, is this reallly how Ryukishi sees his audience? Has the fanbase broken him? Does he actually hate us now? Yeah, that one got me crying. I don’t really interpret that scene that way anymore, but when it first came in, it got me good.